000			02543nam a22002057a 4500
005			20240530112236.0
008			240530b |||||||| |||| 00| 0 eng d
022			_a0021-9584
100			_aSoong, Ronald
245			_aStructures Frozen in Time _b: Application of Basic Cryogenic Electron Microscopy in an Undergraduate Lab (Journal Article)
260			_aWashington DC _b:American Chemical Society _c,2023
300			_a4423–4429p.
440			_aJournal of Chemical Education _vVolume 100, 2023 - Issue 11, November 2023
505			_a***______{For Hard Copy, Please visit Library.}________***
520			_aAbstract: Cryogenic electron microscopy (cryo-EM) is a powerful technique capable of characterizing large protein complexes that are otherwise impossible to characterize using traditional crystallography methods. Cryo-EM has played a pivotal role in our understanding of the structure–function relationship of the spike proteins on the COVID-19 viral capsule, allowing for the rapid development of therapeutics and vaccines in our quest to end the COVID-19 pandemic. The relevance of cryo-EM to this global event advocates for its incorporation into undergraduate chemistry curricula. In fact, highlighting the importance and applicability of a technique to students allows for a better retention of knowledge. Therefore, in this laboratory exercise, we introduce the concepts and application of cryo-EM as a structural characterization tool in an upper-year undergraduate class. Furthermore, with the aid of three-dimensional (3D) printing, the concepts of tomography are introduced, allowing students to understand the construction of a 3D model from a collection of 2D images through cryo-EM. According to student responses, this lab module was well-received, and the 3D-printed model indeed added to their learning experience. While cryo-EM is a specialized technique that may not be available to some educational institutions, those with access should consider its incorporation into their curriculum, thereby exposing students to a breadth of structural characterization techniques that broaden their learning experience.
650			_aUpper-Year Undergraduate/General | Laboratory Equipment/Apparatus| Analytical Chemistry| Collaborative/Cooperative Learning| Laboratory Computing/Interfacing
700			_aFiddes, Lindsey | Pellizzari, Jacob | Downey, Katelyn | Bastawrous, Monica | Adamo, Antonio | Simpson, Andre | Luk, Vivienne
856			_uhttps://doi.org/10.1021/acs.jchemed.3c00359
942			_cPER
999			_c45960 _d45959