02312nam a22002057a 4500 20240117155358.0 240116b ||||| |||| 00| 0 eng d 0021-9584 He, Xiangli An Enzyme Kinetics-Based Laboratory Module Aimed at Introducing Undergraduates to High-Throughput Drug Screening (Journal Article) Washington DC : American Chemical Society , 2023 3841–3848p. Journal of Chemical Society , Volume 100: Number 10, October 2023 ***______{For Hard Copy, Please visit Library.}________*** Abstract: Enzymes provide attractive targets for drug development. Quantitative analysis of enzyme reactions can lead to rational drug discovery. For undergraduates majoring in biochemistry or pharmacy and engaged in drug discovery-related work, it is essential to acquire a comprehensive understanding of enzyme kinetics, including experimental methods and the fundamental principles of drug screening. Being overexpressed in cancer, MTHFD2 is an emerging anticancer drug target. Herein, we used MTHFD2 to develop a high-throughput drug screening laboratory course based on enzyme kinetics for upper-division undergraduate students. The learning activities that were designed for students included Escherichia coli-based protein expression, protein purification, enzyme activity assay, setting up a high-throughput drug screening assay, and data analysis. The main purpose of this one-week laboratory education course is to provide students with a better understanding of enzyme kinetics, high-throughput drug screening and to help them grasp the relationship between enzyme kinetics and drug discovery. This hands-on course provides students an opportunity to learn how theoretical knowledge is applied in real practice. Gather| Gather.town| Avatar| Escape Room| Online Escape Room| Online Learning| Learning Platform| Learning Community| Social Interactions| Pandemic| Remote Learning| Laboratory Class| Practical| Collaborative Learning| Problem Solving Metaverse| Social Constructivism Huang, Ying | Xu, Zhongyu | Liu, Xi | Xu, Zhaomin | Ma, Lei | Zhao, Yuzheng | Huang, Jin https://doi.org/10.1021/acs.jchemed.3c00162 PER 0 0 0 0 RIEBPL RIEBPL 2024-01-17 0 2024-01-17 00:00:00 2024-01-17 PER 45327 45326