01804nam a22002537a 4500 20231106093929.0 231030b ||||| |||| 00| 0 eng d 0021-9584 RIEBPL Library 540.7 Yu Liu et al... Separation of Fluorescent Protochlorophyllide from Green Pumpkin Seed Using Column Chromatography (Journal Article) Washington, United States :American Chemical Society ,January 10, 2023 312-315 p. American Chemical Society, Volume 100, Issue 1 ***______{For Hard Copy, Please visit Library.}________*** Abstract- A new organic chemistry experiment aimed to separate the chlorophyll precursor in plant seeds by column chromatography is described. The primary pedagogical goal in developing this experiment is to establish a simple and interesting chromatographic separation to demonstrate basic concepts and the standard procedure for this important lab technique. The experimental procedure is straightforward and is therefore suitable for college students who are new to organic laboratory. A unique feature of this experiment is that although the target compound is not directly visible in the column, it can be revealed by inducing fluorescence with a handheld UV device. This intriguing laboratory activity, chasing the red fluorescent band while running the column, keeps students engaged in learning column chromatography from this experiment. Second-Year Undergraduate Organic Chemistry Hands-On Learning Chromatography https://doi.org/10.1021/acs.jchemed.2c00652 PER 0 0 0 0 RIEBPL RIEBPL 2023-11-06 0 540.7 2023-11-06 00:00:00 2023-11-06 PER 44686 44685