TY  - BOOK
AU  - Arun Richard Chandrasekaran
TI  -  DNA Nanotechnology in the Undergraduate Laboratory: Electrophoretic Analysis of DNA Nanostructure Biostability: (Journal Article) 
T2  - American Chemical Society, Volume 100, Issue 1
SN  - 0021-9584
U1  - 540.7 
PY  - 0000///,January 102023///
CY  - Washington, United States
PB  - :American Chemical Society
KW  - Upper-Division Undergraduate
KW  -  Biochemistry Interdisciplinary/Multidisciplinary
KW  -  Hands-On Learning/Manipulatives
KW  -  Electrophoresis Molecular Properties/Structure
KW  -  Nanotechnology Nucleic Acids/DNA/RNA
KW  - Undergraduate Research Biophysical
KW  - Chemistry DNA Nanotechnology
N1  - ***______{For Hard Copy, Please visit Library.}________***
N2  - Abstract-

 The field of DNA nanotechnology has grown rapidly in the past decade and has expanded to multiple laboratories. While lectures in DNA nanotechnology have been introduced in some institutions, laboratory components at the undergraduate level are still lacking. Undergraduate students predominantly learn about DNA nanotechnology through their involvement as interns in research laboratories. The DNA nanostructure biostability analysis experiment presented here can be used as a hands-on introductory laboratory exercise for discussing concepts in DNA nanotechnology in an undergraduate setting. This experiment discusses biostability, gel electrophoresis and quantitative analysis of nuclease degradation of a model DNA nanostructure, the paranemic crossover (PX) DNA motif. The experiment can be performed in a chemistry, biology, or biochemistry laboratory with minimal costs and can be adapted in undergraduate institutions using the instructor and student manuals provided here. Laboratory courses based on cutting edge research not only provide students a direct hands-on approach to the subject, but also can increase undergraduate student participation in research. Moreover, laboratory courses that reflect the increasingly multidisciplinary nature of research add value to undergraduate education.





































UR  - https://doi.org/10.1021/acs.jchemed.2c00656
ER  -