Separation of Fluorescent Protochlorophyllide from Green Pumpkin Seed Using Column Chromatography (Journal Article)

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Abstract-

A new organic chemistry experiment aimed to separate the chlorophyll precursor in plant seeds by column chromatography is described. The primary pedagogical goal in developing this experiment is to establish a simple and interesting chromatographic separation to demonstrate basic concepts and the standard procedure for this important lab technique. The experimental procedure is straightforward and is therefore suitable for college students who are new to organic laboratory. A unique feature of this experiment is that although the target compound is not directly visible in the column, it can be revealed by inducing fluorescence with a handheld UV device. This intriguing laboratory activity, chasing the red fluorescent band while running the column, keeps students engaged in learning column chromatography from this experiment.