Structures Frozen in Time (Record no. 45960)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02543nam a22002057a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240530112236.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 240530b |||||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| ISSN | 0021-9584 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Soong, Ronald |
| 245 ## - TITLE STATEMENT | |
| Title | Structures Frozen in Time |
| Remainder of title | : Application of Basic Cryogenic Electron Microscopy in an Undergraduate Lab (Journal Article) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication | Washington DC |
| Name of publisher | :American Chemical Society |
| Year of publication | ,2023 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 4423–4429p. |
| 440 ## - SERIES STATEMENT/ADDED ENTRY--TITLE | |
| Title | Journal of Chemical Education |
| Volume number/sequential designation | Volume 100, 2023 - Issue 11, November 2023 |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | ***______{For Hard Copy, Please visit Library.}________***<br/><br/> |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Abstract: Cryogenic electron microscopy (cryo-EM) is a powerful technique capable of characterizing large protein complexes that are otherwise impossible to characterize using traditional crystallography methods. Cryo-EM has played a pivotal role in our understanding of the structure–function relationship of the spike proteins on the COVID-19 viral capsule, allowing for the rapid development of therapeutics and vaccines in our quest to end the COVID-19 pandemic. The relevance of cryo-EM to this global event advocates for its incorporation into undergraduate chemistry curricula. In fact, highlighting the importance and applicability of a technique to students allows for a better retention of knowledge. Therefore, in this laboratory exercise, we introduce the concepts and application of cryo-EM as a structural characterization tool in an upper-year undergraduate class. Furthermore, with the aid of three-dimensional (3D) printing, the concepts of tomography are introduced, allowing students to understand the construction of a 3D model from a collection of 2D images through cryo-EM. According to student responses, this lab module was well-received, and the 3D-printed model indeed added to their learning experience. While cryo-EM is a specialized technique that may not be available to some educational institutions, those with access should consider its incorporation into their curriculum, thereby exposing students to a breadth of structural characterization techniques that broaden their learning experience. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Upper-Year Undergraduate/General | Laboratory Equipment/Apparatus| Analytical Chemistry| Collaborative/Cooperative Learning| Laboratory Computing/Interfacing |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fiddes, Lindsey | Pellizzari, Jacob | Downey, Katelyn | Bastawrous, Monica | Adamo, Antonio | Simpson, Andre | Luk, Vivienne |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://doi.org/10.1021/acs.jchemed.3c00359 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Periodicals |
| Lost status | Damaged status | Home library | Current library | Date acquired | Koha item type |
|---|---|---|---|---|---|
| RIE BPL Library | RIE BPL Library | 30.05.2024 | Periodicals |