In Vitro Binding of Platinum Anticancer Drugs to DNA Observed Directly by Polyacrylamide Gel Electrophoresis (Record no. 45291)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02516nam a22002057a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240116154353.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 240116b ||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| ISSN | 0021-9584 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Naff, Keira D. |
| 245 ## - TITLE STATEMENT | |
| Title | In Vitro Binding of Platinum Anticancer Drugs to DNA Observed Directly by Polyacrylamide Gel Electrophoresis |
| Remainder of title | : A Multiweek Undergraduate Laboratory Investigation (Journal Article) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication | Washington DC |
| Name of publisher | : American Chemical Society |
| Year of publication | , 2023 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 3576–3583p. |
| 440 ## - SERIES STATEMENT/ADDED ENTRY--TITLE | |
| Title | Journal of Chemical Society |
| Volume number/sequential designation | , Volume 100: Number 9, September 2023 |
| 505 ## - FORMATTED CONTENTS NOTE | |
| Formatted contents note | ***______{For Hard Copy, Please visit Library.}________***<br/><br/> |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Abstract: Structure–activity relationships are foundational concepts in biochemistry and drug design. Despite their importance, it can be difficult to create and execute laboratory activities that allow students to directly observe the interactions between small-molecule drugs and their biomolecular targets. Here, we report an activity that investigates the binding of well-known platinum drugs to short, double-stranded DNA hairpin structures. Students chose multiple Pt drugs to investigate, along with one or more different experimental conditions, performed the Pt–DNA binding reactions, and then characterized the samples by denaturing polyacrylamide gel electrophoresis. Then, they rationalized their gel data on the basis of the structures of the Pt drugs and of double-stranded DNA. In addition to experimentally observing Pt–DNA interactions, students used molecular visualization software to identify other drug interactions with nucleic acids using structures obtained from the Protein Data Bank to determine their mode of binding. The activity was performed by upper-level undergraduate biochemistry students over 3 weeks and uses inexpensive equipment commonly found in any standard undergraduate biochemistry teaching laboratory. It affords an excellent opportunity for students to manipulate and characterize macromolecular structures and directly connect these experimental observations to important concepts in drug chemistry, bioinorganic chemistry, and chemical biology. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Upper-Division Undergraduate| Biochemistry| Laboratory Instruction| Hands-On Learning/Manipulatives| Inquiry-Based/Discovery Learning| Drugs/Pharmaceuticals| Nucleic Acids/DNA/RNA |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tyler, Jeri L. | Hamza, Dima H. | White, Jonathan D. |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://doi.org/10.1021/acs.jchemed.3c00322 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Periodicals |
| Lost status | Damaged status | Home library | Current library | Date acquired | Koha item type |
|---|---|---|---|---|---|
| RIE BPL Library | RIE BPL Library | 16.01.2024 | Periodicals |